Blu Mankuma has the distinct honor of being the first male voice actor in the history of any iteration of the franchise to voice a main female character; Fluttershy was nicknamed Flutterguy because of this.
Near the end when the ponies are worried they crushed Applejack in the bath, Pinkie emits her long gasp from "Friendship is Magic, part 1".
When confronting Zecora about the Apple Bloom soup and the curse on the Mane 6, Rainbow Dash's halter keeps disappearing and reappearing.
The nicknames Spike gives to each of the affected ponies are: Hairity (Rarity), Rainbow Crash (Rainbow Dash), Spitty Pie (Pinkie Pie) Apple-Teeny (Applejack), Flutterguy (Fluttershy), and Twilight Flopple (Twilight Sparkle).
In Africa, women would wear these gold necklaces to extend their necks.