"The Great and Powerful Trixie" refers to herself as such or is called so by other characters a total of 20 times during the episode.
This is the first episode where one or a few of the main characters doesn't have a single line of dialogue. Pinkie Pie and Fluttershy only appear sporadically in the crowd.
This episode shows that Rainbow Dash is NOT immune to motion-sickness, despite her acrobatic-routines, although her nausea could have come from Trixie catching her off-guard.
The original episode script called for a boastful male unicorn, but Chris Savino would later re-imagine the character as female.
Derpy Hooves, the fan-favorite pegasus with crazy eyes, can be seen several times: flying over the audience at 02:19 and a few times in the background between 18:04 and 19:15.