In Arabic numerals, the Roman numeral formula on the sarcophagus reads 2 to the 11th power minus (23 times 89), which is 2048 minus 2047, or 1.
After the crew decide to go to Rome, Hermes asks "didn't we used to be a delivery company?", a reference to the fact that Planet Express has not made any known deliveries in quite some time.
The phrase "My God!" is uttered a total of ten times in this episode. Amy, Leela, Dr. Zoidberg, and Fry each say it once, and Professor Farnsworth says it six times.
Niccolò Machiavelli, an Italian writer who is considered one of the main founders of modern political science, faked his own death. Animatronio does it twice in this episode. Like Leonardo da Vinci, Machiavelli is considered a good example of the Renaissance Man.
In "Spanish Fry" (2003), Fry sets up a pup tent, using a cast-iron frying pan to hammer nail-like spikes into the ground, reinforcing the idea that he does not understand the concept of using a hammer.