At the end, when the mummy chases after Luigi, the color of the princess's dress are purple. Then on the closing shot, the princess's dress is back to her normal colors.
Mario's mustache is brown instead of black when he's in bed at the beginning.
As Hip and Hop Koopa run past the Mario brothers, Toad, and the Princess with the Mummy Case they stole, Hip and Hop's heads are the same tan color as their skin instead of green and Hop's hair is all yellow instead of multi-colored.
As Luigi, the princess, and Toad are chasing Hip and Hop with the stolen Mummy Case, Hip and Hop have changed positions while carrying the case where Hip is in the back and Hop in the front.
When King Koopa, Hip, and Hop arrive at the bottom of the staircase to the tower after being alerted by the Goombas, Hip and Hop's heads are brown instead of green.
In the shot of the Mummy carrying Mario and running towards the pyramid, the ground starts to show as the camera tilts down. In one of the frames, part of the ground on the left side disappears and reappears.