The title of the show was originally "Studio 7 on the Sunset Strip". The title was changed after it was discovered that there was a short-lived WB game show by the name of "Studio 7" (2004).
Matthew Perry originally turned down the role of Matt Albie but Aaron Sorkin did not want to have anyone else play the part and apparently would not take "No" for an answer. Perry reconsidered and decided to jump on board.
Immediately above the countdown board in Matt's office is a black-on-white strip bearing the legend "Time flies like an arrow, fruit flies like a banana. G. Marx"
The character of Harriet Hayes is loosely modeled after creator Aaron Sorkin's former girlfriend, Kristin Chenoweth.
The episode title card and opening credits use the same font as "The West Wing" (1999), another show written by Aaron Sorkin.
The law firm of Gage Whitney Pace, which employed Kari Matchett's character, Mary Tate, was also the law firm that Rob Lowe's character on "The West Wing" (1999), Sam Seaborn, worked at before joining the Bartlet administration.
Timothy Busfield plays Cal Shanley, the director for Studio 60. He actually directed 5 episodes of the series. Likewise, Bradley Whitford, who plays Danny Tripp, a feature film director, directed the final episode of the series.
The show's production logo features a picture of a shoe with the word "money" underneath. "Shoe Money Tonight" was the title of episode 10 in season 1 of Aaron Sorkin's show "Sports Night" (1998). It is a catch phrase that Dana Whitaker uses while playing poker with her boss Isaac Jaffe.
There are a number of set-dressing items from Aaron Sorkin's "The West Wing" (1999) on display in various parts of the Studio 60 set, including the "NO TOURS BEYOND THIS POINT" sign, and the Pirates of Penzance poster on display in Matt Albie's office.