Three of the bikini girls from the bikini machine were Playboy playmates.
In a 1987 interview with David Del Valle, Vincent Price said of this film: "It could have been fun, but they cut all the music out."
Portraits of Goldfoot's ancestors include likenesses of various Vincent Price movie characters, including Verden Fell from The Tomb of Ligeia (1964) and Roderick Usher from House of Usher (1960).
The film had a moderate box-office success in the US, but was a big hit in Italy. The sequel, Dr. Goldfoot and the Girl Bombs (1966), was shot in Italy, with Italian director Mario Bava and Italian comedy duo Franco Franchi and Ciccio Ingrassia.
The dungeon and torture sequence incorporates long shots of Vincent Price lifted from the The Pit and the Pendulum (1961). The torture chamber art and set direction was by the same art director, Daniel Haller, and these scenes evoked his work on other Edgar Allan Poe adaptations for Roger Corman, which also starred Vincent Price.
Annette Funicello: a girl in the dungeon. Only her head and hands are visible in the pillory, which was designed to conceal her pregnant belly.
Alberta Nelson: the gruff-voiced robot #12 reject. Nelson played one of the Rat Pack motorcyclists in the Beach Party movies.
Harvey Lembeck: a motorcycle thug in the dungeon. Implicitly, he is Eric Von Zipper, his regular character from the Beach Party movies.