This is the first 1940 Donald Duck cartoon to feature Spike the Bee who's a rival of Donald Duck. When Donald Duck teased him, he would get his revenge by attempting to sting Donald Ducks tail feathers which he finally did at the end.
Spike the Bee would be in later Donald Duck Cartoons such as "Inferior Decorator" 1948, "Honey Harvester" 1949, "Slide, Donald, Slide", 1949. "Bee at the Beach" 1950 and "Let's Stick Together" 1952 always attempting to sting Donald Ducks tail feathers which Spike the Bee does in the end.